Day 358 of #LMB365 is a Christmas tree composed of Hela cells ectopically expressing golgin-97 at the mitochondria, in green, and co-stained with vesicle cargo proteins in red and blue. Golgin-97 is a peripheral membrane protein that localises to the trans-Golgi via a C-terminal GRIP domain. By replacing the GRIP domain with a mitochondrial targeting sequence we can re-localise golgin-97 from the Golgi to the mitochondria and then, using immunofluorescence, see what happens to the localisation of other proteins in the cell. Using this assay Sean Munro’s group in the Cell Biology Division has shown that Golgin-97 is a vesicle tether that captures endosomally derived vesicles via its N-terminus. The ‘baubles’ are labelled with the cis-Golgi marker gm130 and the ‘star’ labelled with the trans-Golgi markers TGN46 and golgin-245.
MRC Laboratory of Molecular Biology
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