The LMB’s new sub-cellular light sheet microscope allows for high speed 3D imaging of cells and small organisms with minimal damage and loss of fluorescent glow. For day 84 of #LMB365 a green laser is focussed to form a thin light sheet of illumination in the centre of the photo. A small silver spoon holds the sample and moves it through the sheet at high speed. Thanks to the gentleness of this technique, scientists can study a range of cells and small organisms for hundreds of time points before the signal is lost.