Day 97 of #LMB365 shows a precious column collection mounted on the wall of a cold room using aluminium holders designed by the LMB’s Director and made in the Technical Instrumentation Workshop. This is an essential part of the preparation of samples for the Structural Studies Division. The columns are filled with special resins that separate the injected protein samples based on their affinity for such resins, or on their charge or size. Dependent on the sample size and purification step, the columns need to be of different diameter, length and volume.