Melina has now relocated her lab to the Max Planck Institute for Biophysical Chemistry, Germany
To become a fertilizable egg, an oocyte has to eliminate half of the chromosomes into a small polar body, because the other half will be contributed by the sperm during fertilization. Errors during chromosome segregation result in aneuploid embryos and thus miscarriages, genetic disorders, or even infertility.
Our aim is to better understand the causes of these defects. We work with mouse oocytes because they are the best model for human oocytes that can be studied with state-of-the-art molecular cell biology and genetic tools. Moreover, we established techniques that allow us to analyze meiotic maturation in live oocytes under physiological conditions at high resolution using quantitative confocal microscopy.
With these powerful tools, we already investigated how the acentrosomal meiotic spindle is assembled and how it is asymmetrically positioned before polar body extrusion.
Our next challenge is to understand how these key steps of oocyte maturation are controlled and how they ensure that the embryo will obtain the correct number of chromosomes.
- Pfender, S.*, Kuznetsov, V.*, Pasternak, M.*, Tischer, T., Santhanam, B., and Schuh, M. (2015)
Live imaging RNAi screen reveals genes essential for meiosis in mammalian oocytes.
Nature 524: (7564):239-42. doi: 10.1038/nature14568
- Holubcová, Z., Blayney, M., Elder, K. and Schuh, M. (2015)
Error-prone chromosome-mediated spindle assembly favors chromosome segregation defects in human oocytes.
Science 348: 1143-1147
- Clift, D. and Schuh, M. (2013)
Restarting life: fertilization and the transition from meiosis to mitosis.
Nature Reviews Molecular Cell Biology, doi: 10.1038/nrm3643
- Holubcová, Z., Howard, G. and Schuh, M. (2013)
Vesicles modulate an actin network for asymmetric spindle positioning
Nature Cell Biology 15: 937-47
- Schuh, M. (2011)
An actin-dependent mechanism for long-range vesicle transport.
Nature Cell Biology 13: 1431-1436
- Pfender, S., Kuznetsov, V., Pleiser, S., Kerkhoff, E. and Schuh, M. (2011)
Spire-type actin nucleators cooperate with formin-2 to drive asymmetric oocyte division.
Current Biology 21: 955-960.
- Schuh, M. and Ellenberg, J. (2007)
Self-organization of MTOCs replaces centrosome function during acentrosomal spindle assembly in live mouse oocytes.
Cell 130: 484-498.
- Dean Clift
- Michal Pasternak
- Thomas Tischer
- Liam Cheeseman