This photo for day 257 of #LMB365 was taken from Hobson’s Park, south-west of the LMB, which is part of the Great Kneighton development.
Image365
LMB 365 – Day 256
KDEL is the sorting signal for luminal ER proteins. In this a classic experiment for day 256 of #LMB365, lysozyme was expressed in COS cells without (left) or with (right) the addition of the sequence SEKDEL, and detected by immunofluorescence. Normally, lysozyme is rapidly secreted and the main pool of intracellular protein is in transit through the Golgi apparatus. Addition of KDEL causes it to accumulate to high levels in the ER. This was the original proof that (SE)KDEL is a sufficient signal for ER retention.
LMB 365 – Day 255
This image for day 255 of #LMB365 shows our Head of Scientific Computing donating the LMB’s old line printer to the Centre for Computing History (CCH) in Cambridge http://www.computinghistory.org.uk/. The printer was bought in 1991 for £8,125 and has been in constant use until very recently… Before graphics terminals became so sophisticated and image processing so automated, the LMB’s line printer gave essential output of large scale images and transforms for manual analysis of helical assemblies, such as tubes of the acetylcholine receptor
LMB 365 – Day 254
Day 254 of #LMB365 showsa reflection of the LMB in the nearby AstraZeneca building at sunset. We wonder whether they can see similar reflections of their building in the glass façade of our building?
LMB 365 – Day 253
As the model organismC. elegansis transparent we can use it to look at subcellular organelles and use fluorescent protein tags to study different processes. This image for day 253 of #LMB365 shows two different organelles labelled with green and magenta, which shows as white if there is co-localisation
LMB 365 – Day 252
Day 252 of #LMB365 shows the LMB’s Research Office who had an awesome team day recently and visited the LMB’s animal facility. They learned more about the work carried out there and how the mice are cared for.