Category Archives: Image365
On day 264 of #LMB365 is an image of the structure of the 30S ribosomal subunit. This structure was first published #OTD in 2000 in a landmark paper in Nature by Venki Ramakrishnan and colleagues. The 30S ribosomal subunit has a crucial role in decoding mRNA by monitoring base pairing between the codon on mRNA and the anticodon on transfer RNA. Venki shared the 2009 @Nobel Prize in Chemistry for his work on determining the ribosome structure. Brian T.Wimberly, Ditlev E.Brodersen, William M.Clemons Jr, Robert J.Morgan-Warren, Andrew P.Carter, Clemens Vonrhein, Thomas Hartsch, V.Ramakrishnan. Structure of the 30S ribosomal subunit. Nature 407(6802):327-339, 21 September 2000
Much of the research at the LMB must be conducted in carefully controlled conditions of temperature, vibration and magnetic fields. The Electronics Workshop works with Group Leaders to detect and analyse extremely small sources of potential interference. This image for day 263of #LMB365 shows a test spectrum createdusing precision sensors and software.
Day 262 of #LMB365 shows a confocal image of a gravid Caenorhabditis elegansworm from Ranya Behbehani in Rebecca Taylor’s lab in the Neurobiology Division. This model organism has been used at the LMB for many years since the initial studies on the genetic regulation of organ development by Sydney Brenner, Bob Horvitz and John Sulston.
Every year or so the LMB takes a group photo of all the current PhD students. Photos in the LMB Archive go back to at least the 1990’s. Day 261 of #LMB365 shows the current cohort posing for this year’s photo – watch this space for the actual photo…
Day 260 of #LMB365 shows Michael Fuller, who was born on this day in 1936. He served the LMB for over 44 years, as apprentice technician, Steward, Laboratory Services Manager and Special Projects Co-ordinator. This photo, taken at Venki Ramakrishnan’s Nobel party in 2009, was the 6th Nobel party organized by Michael
Drosophila Melanogaster is a key model organism used across the LMB to study fundamental aspects of biology. This image for day 259 of #LMB365 shows the dorsal portion of the fly thorax, called the notum, imaged by Scanning Electron Microscopy (SEM) taken by Emmanuel Derivery in the LMB’s Cell Biology Division. In normal conditions (right-hand side), the notum is covered with hair, which are mechanosensory organs, and constitute a well-established system to study cell fate determination following asymmetric cell division. When the mechanisms of cell fate determination are compromised, for instance in mutant conditions (left-hand side), these hairs disappear. Such genetic studies are thus helpful to further our understanding of the molecular mechanisms of asymmetric cell fate determination during stem cell division
On day 258 of#LMB365ina break between experiments, LMB scientists get competitive at table football in one of the communal spaces across the building. The table was purchased by crowd-funding within the LMB over 5 years ago and is still going strong!
This photo for day 257 of #LMB365 was taken from Hobson’s Park, south-west of the LMB, which is part of the Great Kneighton development.
KDEL is the sorting signal for luminal ER proteins. In this a classic experiment for day 256 of #LMB365, lysozyme was expressed in COS cells without (left) or with (right) the addition of the sequence SEKDEL, and detected by immunofluorescence. Normally, lysozyme is rapidly secreted and the main pool of intracellular protein is in transit through the Golgi apparatus. Addition of KDEL causes it to accumulate to high levels in the ER. This was the original proof that (SE)KDEL is a sufficient signal for ER retention.
This image for day 255 of #LMB365 shows our Head of Scientific Computing donating the LMB’s old line printer to the Centre for Computing History (CCH) in Cambridge http://www.computinghistory.org.uk/. The printer was bought in 1991 for £8,125 and has been in constant use until very recently… Before graphics terminals became so sophisticated and image processing so automated, the LMB’s line printer gave essential output of large scale images and transforms for manual analysis of helical assemblies, such as tubes of the acetylcholine receptor