Electron Microscopy

Electron Cryo-microscopy Course 2023

Influential publications in the historical development of cryo-EM – Richard Henderson

Electron cryomicroscope hardware: past, present, and future – Chris Russo

Specimen preparation for cryo-EM – Katerina Naydenova

Electron cryo-microscopy detectors – Greg McMullen

Cryo-EM Data Collection – Giuseppe Cannone

Cryo-EM Image Processing – Sjors Scheres

Processing difficult datasets in cryo-EM – Kelly Nguyen

Modelling using Cryo-EM Data: A Review of the Problems and Software (including Coot) – Paul Emsley

Cryo-electron tomography – Tanmay Bharat

FIB milling specimens for electron cryo-microscopy – Vicky Hale

Below are recordings of the LMB’s 2017 Cryo-microscopy course. Full copies of the lecture presentations can be found below the videos.

Cryo-EM17 lecture 01: Cryo-EM: past, present & future – Richard Henderson

Cryo-EM17 lecture 02: Microscopy physics and optics – Chris Russo

Cryo-EM17 lecture 03: Image formation, Fourier analysis, CTF – Paula da Fonseca

Cryo-EM17 lecture 04: Data acquisition – Christos Savva

Cryo-EM17 lecture 05: Sample preparation – Lori Passmore

Cryo-EM17 lecture 06: Image refinement in 2D and 3D – Sjors Scheres

Cryo-EM17 lecture 07: Data processing strategy – Rafael Fernandez-Leiro

Cryo-EM17 lecture 08: Atomic modeling & validation – Alan Brown

Cryo-EM17 lecture 09: Tomography – John Briggs

Cryo-EM17 lecture 10: Local setup and training – Shaoxia Chen & Giuseppe Cannone

Cryo-EM14 lecture 1: Course intro with historical perspective - Tony Crowther

Cryo-EM14 lecture 2: Image formation, fourier analysis and CTF theory - Sjors Scheres

Cryo-EM14 lecture 3: Electron microscope physics and optics - Chris Russo

Cryo-EM14 lecture 4: Sample preparation - Lori Passmore

Cryo-EM14 lecture 5: Initial data analysis - Paula da Fonseca

Cryo-EM14 lecture 6: Image refinement in 2d+3d - Sjors Scheres

Cryo-EM14 lecture 7: Tomography and subtomography averaging - Tanmay Bharat

Cryo-EM14 lecture 8: Resolution, sharpening, validation - Richard Henderson

Cryo-EM14 lecture 9: Modelling in cryo EM maps - Leifu Chang and Alan Brown

Cryo-EM14 lecture 10: Local set up and training - Shaoxia Chen and Christos Savva