Electron Microscopy

Electron Cryo-microscopy Course 2017

Below are recordings of the LMB’s 2017 Cryo-microscopy course. Full copies of the lecture presentations can be found below the videos.

Cryo-EM17 lecture 01: Cryo-EM: past, present & future - Richard Henderson

Cryo-EM17 lecture 02: Microscopy physics and optics - Chris Russo

Cryo-EM17 lecture 03: Image formation, Fourier analysis, CTF - Paula da Fonseca

Cryo-EM17 lecture 04: Data acquisition - Christos Savva

Cryo-EM17 lecture 05: Sample preparation - Lori Passmore

Cryo-EM17 lecture 06: Image refinement in 2D and 3D - Sjors Scheres

Cryo-EM17 lecture 07: Data processing strategy - Rafael Fernandez-Leiro

Cryo-EM17 lecture 08: Atomic modeling & validation - Alan Brown

Cryo-EM17 lecture 09: Tomography - John Briggs

Cryo-EM17 lecture 10: Local setup and training - Shaoxia Chen & Giuseppe Cannone

Cryo-EM17 Lecture 01 Past Present Future (88.6 MB)
Cryo-EM17 Lecture 02 Physics Optics (29.0 MB)
Cryo-EM17 Lecture 03 Fourier Analysis (4.7 MB)
Cryo-EM17 Lecture 04 Data Acquisition (56.0 MB)
Cryo-EM17 Lecture 05 Sample Preparation (185.9 MB)
Cryo-EM17 Lecture 06 Refinement (9.8 MB)
Cryo-EM17 Lecture 07 Processing Strategy (140.8 MB)
Cryo-EM17 Lecture 08 Modelling (34.6 MB)
Cryo-EM17 Lecture 09 Tomography (4.9 MB)

Cryo-EM14 lecture 1: Course intro with historical perspective - Tony Crowther

Cryo-EM14 lecture 2: Image formation, fourier analysis and CTF theory - Sjors Scheres

Cryo-EM14 lecture 3: Electron microscope physics and optics - Chris Russo

Cryo-EM14 lecture 4: Sample preparation - Lori Passmore

Cryo-EM14 lecture 5: Initial data analysis - Paula da Fonseca

Cryo-EM14 lecture 6: Image refinement in 2d+3d - Sjors Scheres

Cryo-EM14 lecture 7: Tomography and subtomography averaging - Tanmay Bharat

Cryo-EM14 lecture 8: Resolution, sharpening, validation - Richard Henderson

Cryo-EM14 lecture 9: Modelling in cryo EM maps - Leifu Chang and Alan Brown

Cryo-EM14 lecture 10: Local set up and training - Shaoxia Chen and Christos Savva