Replication can be arrested by DNA damage or by naturally occurring DNA secondary structures. We are particularly interested in translesion synthesis (TLS), which is mediated by specialised DNA polymerases and which is required for replication of both DNA damage and a particular secondary structure called a G quadruplex. Although TLS is potentially mutagenic it plays a critical role in normal cells and understanding its control is central to understanding cell transformation.
We study these processes using the powerful combination of vertebrate somatic cell genetics coupled with biochemical, biophysical and advanced optical microscopy techniques to monitor the molecular choreography of proteins and DNA at sites of stalled replication.
The green and red portions each reflect 20 minutes of DNA synthesis. DNA damage has been added
during the second (red) labelling period in the lower panel resulting in replication fork stalling.
- Frey, A., Listovsky, T., Guilbaud, G., Sarkies, P. and Sale, J.E. (2014)
Histone H3.3 is required to maintain replication fork progression after UV damage
Current Biology 24: 2195-2201.
- Schiavone, D., Guilbaud, G., Murat, P., Papadopoulou, C., Sarkies, P., Prioleau, M.-N., Balasubramanian, S. and Sale, J.E. (2014)
Determinants of G quadruplex-mediated epigenetic instability in REV1-deficient cells.
EMBO Journal 33: 2507-2520.
- Sale J.E. (2013)
Translesion synthesis and mutagenesis in eukaryotes.
Cold Spring Harbor Perspectives in Biology 5: (3):a012708.
- Sarkies, P. and Sale, J.E. (2012)
Cellular epigenetic memory and cancer.
Trends in Genetics 28: 118-127.
- Guillaume Guilbaud
- Sasa Svikovic
- Daniela Peris
- Benedicte Recolin
- Leticia Koch-Lerner
- Cara Eldridge