• Skip to primary navigation
  • Skip to main content
  • Skip to primary sidebar
MRC Laboratory of Molecular Biology

MRC Laboratory of Molecular Biology

One of the world's leading research institutes, our scientists are working to advance understanding of biological processes at the molecular level - providing the knowledge needed to solve key problems in human health.

  • Home
  • About LMB
  • Research
  • Research Groups
  • Students
  • Recruitment
  • Life at the LMB
  • Achievements
  • News & Events
Home > Research Leaders > T to Z > Joseph Yeeles
Joseph Yeeles pnac

Joseph Yeeles

Mechanisms of chromosome replication

jyeeles@mrc-lmb.cam.ac.uk

Precise chromosome replication is essential for maintaining genome stability, yet the process itself is inherently risky. Duplex DNA must be transiently separated into unstable single-strands; a vast number of DNA bases must be accurately copied; and replication forks frequently encounter protein barriers and unrepaired DNA damage that can stall and even derail the replication machinery (replisome).

Our lab is interested in mechanisms of chromosome replication, and how these mechanisms contribute to faithful genome duplication.

Regulated assembly of a minimal eukaryotic replisome with purified proteins
Figure modified from Yeeles, J.T., Deegan, T.D., Janska, A., Early, A. and Diffley, J.F. (2015) Nature 519(7544): 431-435, with permission from J Diffley.

The eukaryotic replisome is a highly complex molecular machine that is assembled from in excess of 30 gene products and which orchestrates the activities of multiple enzymatic components. To study this remarkable machine in action our lab reconstitutes replication in vitro using purified proteins. This highly defined experimental system, used in conjunction with genetic approaches and replication proficient cell-free extracts, enables us to investigate fundamental aspects of replisome biology.

Selected Papers

  • Baretić, D., Jenkyn-Bedford, M., Aria, V., Cannone, G., Skehel, M., Yeeles, JTP. (2020)
    Cryo-EM structure of the Fork Protection Complex bound to CMG at a replication fork.
    Molecular Cell 78: 1-15.
  • Guilliam, TA., Yeeles, JTP. (2020)
    Reconstitution of translesion synthesis reveals a mechanism of eukaryotic DNA replication restart.
    Nature Structural and Molecular Biology, Epub ahead of print.
  • Aria V, Yeeles JTP. (2018)
    Mechanism of Bidirectional Leading-Strand Synthesis Establishment at Eukaryotic DNA Replication Origins.
    Mol Cell. 73: 199-211 e10.
  • Taylor MRG, Yeeles JTP. (2018)
    The Initial Response of a Eukaryotic Replisome to DNA Damage.
    Mol Cell. 70(6): 1067-1080.
  • Yeeles JTP, Janska A, Early A, Diffley JFX. (2017)
    How the Eukaryotic Replisome Achieves Rapid and Efficient DNA Replication.
    Mol Cell. 65(1): 105-116.
  • Yeeles, J.T., Deegan, T.D., Janska, A., Early, A. and Diffley, J.F. (2015)
    Regulated eukaryotic DNA replication origin firing with purified proteins.
    Nature 519(7544): 431-435.

Group Members

  • Valentina Aria
  • Yasemin Baris
  • Emma Fletcher
  • Tom Guilliam
  • Michael Hodskinson
  • Michael Jenkyn-Bedford
  • Morgan Jones
  • Rose (Emma) Westhorpe

Primary Sidebar

Research Leaders

  • A to G
    • Radu Aricescu
    • David Barford
    • Buzz Baum
    • Anne Bertolotti
    • Mariann Bienz
    • John Briggs
    • Simon Bullock
    • Albert Cardona
    • Andrew Carter
    • Jason Chin
    • Gerry Crossan
    • Emmanuel Derivery
    • Benjamin Falcon
    • Michel Goedert
    • Julian Gough
    • Ingo Greger
  • H to M
    • Michael Hastings
    • Ramanujan Hegde
    • Richard Henderson
    • Philipp Holliger
    • Leo James
    • Gregory Jefferis
    • Patrycja Kozik
    • Madeline Lancaster
    • Jan Löwe
    • Kate McDole
    • Andrew McKenzie
    • Harvey McMahon
    • Liz Miller
    • Sean Munro
    • Garib Murshudov
  • N to S
    • Kiyoshi Nagai (1949-2019)
    • David Neuhaus
    • Kelly Nguyen
    • John O’Neill
    • Lori Passmore
    • Venki Ramakrishnan
    • Felix Randow
    • Jing Ren
    • Katja Röper
    • Christopher Russo
    • Julian Sale
    • William Schafer
    • Sjors Scheres
    • Marta Shahbazi
    • John Sutherland
  • T to Z
    • Chris Tate
    • Rebecca Taylor
    • Marco Tripodi
    • Ana Tufegdzic Vidakovic
    • Roger Williams
    • Joseph Yeeles
    • Marta Zlatic
  • Emeritus
    • Brad Amos
    • Tony Crowther
    • Phil Evans
    • Alan Fersht
    • Michael Gait
    • Rob Kay
    • John Kendrick-Jones
    • John Kilmartin
    • Peter Lawrence
    • Andrew Leslie
    • Hugh Pelham
    • Daniela Rhodes
    • Murray Stewart
    • Andrew Travers
    • Nigel Unwin
    • Greg Winter
  • LMB Fellows
  • Molecular Immunity Unit

Search

  • Privacy & Cookies
  • Contact Directory
  • Freedom of Information
  • Site Map
Find Us
©2021 MRC Laboratory of Molecular Biology,
Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK. 01223 267000

The MRC is part of UK Research and Innovation

[ Placeholder content for popup link ] WordPress Download Manager - Best Download Management Plugin