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Home > Research Leaders > A to G > Matteo Allegretti
Matteo Allegretti ss

Matteo Allegretti

The macromolecular basis of compartment remodelling


Personal group site

The cell is the simplest living metabolic entity. Organelles are cellular compartments specialized in a certain function. The nucleus is the essential cell compartment containing the genetic information. Our research aims to understand the molecular basis of how the nucleus is remodelled in shape during differentiation processes or due to environmental cues. Defects or anomalies during remodelling are directly involved in the biogenesis of diseases like infertility, developmental disorders and cancer metastatic progression, therefore a molecular understanding of such processes is needed to lead towards new therapeutic targets.

Allegretti_fig

The nucleus is a very elastic organelle whose shape is regulated by the interplay between several macromolecular complexes that assemble and disassemble in response to physiological stimuli. Until now the understanding of such processes in molecular details is limited. Our lab aims to investigate the structural organization and conformational adaptations of protein complexes which shape the nucleus, and whose mutations are linked to diseases. Macromolecules include cytoskeleton and nuclear envelope proteins.

figs 2 and 3 Allegretti

Furthermore, our lab aims to develop methods which bridge the fields of structural and cell biology, in particular to integrate electron microscopy, light microscopy and molecular modelling to investigate the structural dynamics of protein interactions in their cellular context.

Selected Papers

  • Zila V., Margiotta E., Turoňová B., Müller T.G., Zimmerli C.E., Mattei S., Allegretti, M., Börner K., Rada J., Müller B., Lusic M., Kräusslich H-G., Beck M. (2021)
    Cone-shaped HIV-1 capsids are transported through intact nuclear pores
    Cell 184(4): 1032-1046.
  • Zimmerli CE, Allegretti M, Rantos V, Goetz SK, Obarska-Kosinska A, Zagoriy I, Halavatyi A, Hummer G, Mahamid J, Kosinski J, Beck M. (2021)
    Nuclear pores dilate and constrict in cellulo.
    Science, eabd9776. doi: 10.1126/science.abd9776.
  • Allegretti, M., Zimmerli, C.E., Rantos, V., Wilfling, F., Ronchi, P., Fung, H.K.H., Lee, C-W., Hagen, W., Turoňová, B., Karius, K., Börmel, M., Zhang, X., Müller, C.W., Schwab, Y., Mahamid, J., Pfander, B,. Kosinski, J., Beck M. (2020)
    In-cell architecture of the nuclear pore and snapshots of its turnover
    Nature 586(7831): 796-800.
  • Lee, C-W., Wilfling, F., Ronchi, P., Allegretti, M., Mosalaganti, S., Jentsch S., Beck M., Pfander, B. (2020)
    Selective autophagy degrades nuclear pore complexes
    Nat Cell Biol. 22(2): 159-166.
  • Thaller, D.J., Allegretti, M., Borah, S., Ronchi, P., Beck, M., Lusk, C.P. (2019)
    An ESCRT-LEM protein surveillance system is poised to directly monitor the nuclear envelope and nuclear transport system
    Elife 8: e45284.
  • Lele , T.P., Dickinson, R.B., Gundersen, G.G. (2018)
    Mechanical principles of nuclear shaping and positioning
    J Cell Biol. 217(10): 3330-3342.
  • Cossio, P., Allegretti, M., Mayer, F., Müller, V., Vonck, J., Hummer, G. (2018)
    Bayesian inference of rotor ring stoichiometry from electron microscopy images of archaeal ATP synthase
    Microscopy (Oxf) 67(5): 266-273.
  • Elosegui-Artola, A., Andreu, I., Beedle, A.E.M., Lezamiz, A., Uroz, M., Kosmalska, A.J., Oria, R., Kechagia, J.Z., Rico-Lastres, P., Le Roux, A-L., Shanahan , C.M., Trepat, X., Navajas, D., Garcia-Manyes, S., Roca-Cusachs, P. (2017)
    Force Triggers YAP Nuclear Entry by Regulating Transport across Nuclear Pores
    Cell 171(6): 1397-1410.
  • Bell, E.S., Lammerding J. (2016)
    Causes and consequences of nuclear envelope alterations in tumour progression
    Eur J Cell Biol. 95(11): 449-464.
  • Denais, C.M., Gilbert, R.M., Isermann, P., McGregor, A.L., te Lindert, M., Weigelin, B., Davidson, P.M., Friedl, P., Wolf, K., Lammerding J. (2016)
    Nuclear envelope rupture and repair during cancer cell migration
    Science 352(6283): 353-8.
  • Allegretti, M., Klusch, N., Mills, D.J., Vonck J., Kühlbrandt, W., Davies, K.M. (2015)
    Horizontal membrane-intrinsic alpha-helices in the stator a-subunit of an F-type ATP synthase
    Nature 521(7551): 237-40.
  • Allegretti, M., Mills, D.J., McMullan, G., Kühlbrandt, W., Vonck J. (2014)
    Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector
    Elife 3: e01963.
  • Gundersen, G.G., Worman, H.J. (2013)
    Nuclear positioning
    Cell 152(6): 1376-89.
  • Mammoto, T., Mammoto, A., Ingber, D.E. (2013)
    Mechanobiology and developmental control
    Annu Rev Cell Dev Biol. 29: 27-61.
  • Fabian, L., Brill, J.A. (2012)
    Drosophila spermiogenesis: Big things come from little packages
    Spermatogenesis 2(3): 197-212.

Group Members

  • Matteo Allegretti
  • Alia dos Santos
  • Thomas Hale

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Research Leaders

  • A to G
    • Matteo Allegretti
    • Radu Aricescu
    • David Barford
    • Buzz Baum
    • Anne Bertolotti
    • Tanmay Bharat
    • Mariann Bienz
    • Simon Bullock
    • Albert Cardona
    • Andrew Carter
    • Jason Chin
    • Gerry Crossan
    • Emmanuel Derivery
    • Michel Goedert
    • Julian Gough
    • Ingo Greger
  • H to M
    • Michael Hastings
    • Ramanujan Hegde
    • Richard Henderson
    • Philipp Holliger
    • Leo James
    • Gregory Jefferis
    • Patrycja Kozik
    • Madeline Lancaster
    • Jan Löwe
    • Kate McDole
    • Andrew McKenzie
    • Harvey McMahon
    • Liz Miller
    • Sean Munro
    • Garib Murshudov
  • N to S
    • Kelly Nguyen
    • John O’Neill
    • Lori Passmore
    • Lalita Ramakrishnan
    • Venki Ramakrishnan
    • Felix Randow
    • Jing Ren
    • Christopher Russo
    • Benjamin Ryskeldi-Falcon
    • Julian Sale
    • William Schafer
    • Sjors Scheres
    • Marta Shahbazi
    • John Sutherland
  • T to Z
    • Chris Tate
    • Marco Tripodi
    • Ana Tufegdžić Vidaković
    • Roger Williams
    • Joseph Yeeles
    • Suyang Zhang
    • Marta Zlatic
  • Emeritus
    • Brad Amos
    • Tony Crowther
    • Phil Evans
    • Alan Fersht
    • Michael Gait
    • Rob Kay
    • John Kendrick-Jones
    • John Kilmartin
    • Peter Lawrence
    • Andrew Leslie
    • David Neuhaus
    • Hugh Pelham
    • Daniela Rhodes
    • Murray Stewart
    • Andrew Travers
    • Nigel Unwin
    • Greg Winter
  • LMB Fellows
  • Molecular Immunity Unit

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