Amyloids in neurodegenerative diseases
Personal group site
Ordered assembly of a small number of proteins within neurons and, in some cases, glia is a feature of neurodegenerative diseases. These proteins form amyloid structures enriched in repetitively stacked beta-strands. Mutations in the genes encoding these proteins lead to assembly and inherited neurodegenerative disease, demonstrating a causal role. Assemblies arise in discrete brain regions, from where they appear to propagate (spread and amplify) within connected brain networks, ultimately leading to neurodegeneration.
We work to understand the molecular basis of protein assembly into amyloid in neurodegenerative disease. Key questions that interest us are: How do assemblies form and propagate? How does this contribute to nerve cell degeneration? Why are only some cell populations in the brain vulnerable? Why does selective vulnerability differ between diseases?
To answer these questions, we combine structural and cell biology, using high-resolution electron microscopy with patient samples and advanced models of assembly in neuronal and glial cells. Electron cryo-microscopy (cryo-EM) of filament structures from human brains revealed that tau assemblies exist as disease-specific conformers, formed from conserved secondary structure motifs with different turn conformations and non-proteinaceous components. We are now studying how these structures relate to selective cell vulnerability by focusing on the molecular interactions between assemblies and brain cells.
- Arseni D, Chen R, Murzin A G, Peak-Chew S Y, Garringer H J, Newell K L, Kametani F, Robinson A C, Vidal R, Ghetti B, Hasegawa M and Ryskeldi-Falcon B. (2023)
TDP-43 forms amyloid filaments with a distinct fold in type A FTLD-TDP
- Yang Y, Arseni D , Zhang W, Huang M , Lövestam S, Schweighauser M, Kotecha A, Murzin A G, Peak-Chew S Y, Macdonald J, Lavenir I, Garringer H J, Gelpi E, Newell K L, Kovacs G G, Vidal R, Ghetti B, Ryskeldi-Falcon B, Scheres S H W, and Goedert M (2022)
Cryo-EM structures of amyloid-β 42 filaments from human brains
Science, pp. 167-172
- Arseni D, Hasegawa M, Murzin AG, Kametani F, Arai M, Yoshida M, Ryskeldi-Falcon B. (2021)
Structure of pathological TDP-43 filaments from ALS with FTLD
Nature, doi: 10.1038/s41586-021-04199-3.
- Falcon B, Zivanov J, Zhang W, Murzin A, Garringer H, Vidal R, Crowther A, Newell K, Ghetti B, Goedert M and Scheres SHW. (2019)
Novel tau filament fold in chronic traumatic encephalopathy encloses hydrophobic molecules.
Nature 568: 420-423.
- Falcon B, Zhang W, Murzin AG, Murshudov G, Garringer HJ, Vidal R,Crowther RA, Ghetti B, Scheres SHW & Goedert M. (2018)
Structures of filaments from Pick’s disease reveal a novel tau protein fold.
Nature 561: 137-140.
- Fitzpatrick AWP, Falcon B, He S, Murzin AG, Murshudov G, Garringer HJ, Crowther RA, Ghetti B, Goedert M & Scheres SHW. (2017)
Cryo-EM structures of tau filaments from Alzheimer’s disease.
Nature 547: 185-190.
- Goedert M, Masuda-Suzukake M & Falcon B. (2017)
Like prions: the propagation of aggregated tau and α-synuclein in neurodegeneration.
Brain 140: 266-278.
- Diana Arseni
- Tiana Behr
- Heidy Chen
- Stephan Tetter