• Skip to primary navigation
  • Skip to main content
  • Skip to primary sidebar
MRC Laboratory of Molecular Biology

MRC Laboratory of Molecular Biology

One of the world's leading research institutes, our scientists are working to advance understanding of biological processes at the molecular level - providing the knowledge needed to solve key problems in human health.

  • Home
  • About LMB
  • Research
  • Research Groups
  • Students
  • Recruitment
  • Life at the LMB
  • Achievements
  • News & Events
Home > Research Leaders > N to S > Felix Randow
pnac

Felix Randow

Cell-autonomous and innate immunity


Personal group site

We are interested in cell autonomous innate immunity, i.e. in the ability of individual cells to defend themselves against infection.

salmonella autophagy pathways
Targeting of anti-bacterial autophagy to a cytosol-invading bacterium through galectin?8?mediated glycan detection on a damaged endomembrane and recruitment of the cargo receptor NDP52.

Cytosolic defence against bacterial invasion

The mammalian cytosol is rich in nutrients but can be colonized by specialized bacteria only. We therefore wish to understand i) how cells succeed in defending their cytosol against most invasion attempts and ii) how professional cytosol dwelling bacteria outwit cellular defences. We are particularly interested in danger receptors that detect breaches in cellular integrity, and in the ubiquitin system that marks invading bacteria as cargo for autophagy receptors.

Regulation of NF-?B and IRF signalling

The defence against pathogens requires profound changes in gene expression that are coordinated by latent transcription factors of the NF-kB and IRF families. We aim to understand how infection activates NF-?B and IRF signalling.

Somatic cell genetics

The genetic analysis of somatic cell lines holds significant potential for our understanding of signal transduction and other cell autonomous traits. We use haploid cells, CRISPR technology, and chemical mutagenesis to isolate genes governing the interaction between pathogens and host cells.

Selected Papers

  • Otten, E.G., Werner, E., Crespillo-Casado, A., Boyle, K.B., Dharamdasani, V., Pathe, C., Santhanam, B., and Randow, F. (2021)
    Ubiquitylation of lipopolysaccharide by RNF213 during bacterial infection
    Nature 594: 111-116.
  • Wandel, M.P., Kim, B.-H., Park, E.-S., Boyle, K.B., Nayak, K., Lagrange, B., Herod, A., Henry, T., Zilbauer, M., Rohde, J., MacMicking, J., Randow, F. (2020)
    Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms
    Nat Immunol 20(8): 1-12.
  • Ellison, C.J., Kukulski, W, Boyle, KB, Munro, S., Randow, F. (2020)
    Transbilayer movement of sphingomyelin precedes catastrophic breakage of enterobacteria-containing vacuoles
    Current Biology 30: 1-10.
  • Ravenhill BJ, Boyle KB, von Muhlinen N, Ellison CJ, Masson GR, Otten EG, Foeglein A, Williams R, Randow F. (2019)
    The cargo receptor NDP52 initiates selective autophagy by recruiting the ULK complex to cytosol-invading bacteria.
    Mol. Cell 74(2): 320-329.
  • Wandel, M., Pathe, C., Werner, E., Ellison, C., Boyle, K., Rohde, J., Randow, F. (2017)
    GBPs inhibit motility of Shigella flexneri but are targeted for degradation by the bacterial ubiquitin ligase IpaH9.8.
    Cell Host and Microbe 22: 5-7-518.
  • Noad, J., Malsburg, von der, A., Pathe, C., Michel, M.A., Komander, D., and Randow, F. (2017)
    LUBAC-synthesized linear ubiquitin chains restrict cytosol-invading bacteria by activating autophagy and NF-κB.
    Nat. Microbiol 2: 17063.
  • Thurston TL, Boyle KB, Allen M, Ravenhill BJ, Karpiyevich M, Bloor S, Kaul A, Noad J, Foeglein A, Matthews SA, Komander D, Bycroft M, Randow F. (2016)
    Recruitment of TBK1 to cytosol-invading Salmonella induces WIPI2-dependent antibacterial autophagy.
    EMBO J. 35: (16):1779-92.
  • Beale, R., Wise, H., Stuart, A., Ravenhill, B.J., Digard, P., and Randow, F. (2014)
    A LC3-interacting motif in the influenza A virus M2 protein is required to subvert autophagy and maintain virion stability.
    Cell Host and Microbe 15: 239–247.
  • Randow, F., MacMicking, J.D., and James, L.C. (2013)
    Cellular self-defense: How cell-autonomous immunity protects against pathogens.
    Science 340: 701–706.
  • Thurston, T.L.M., Wandel, M.P., Muhlinen, Von, N., Foeglein, Á., and Randow, F. (2012)
    Galectin 8 targets damaged vesicles for autophagy to defend cells against bacterial invasion.
    Nature 482: 414–418.
  • Muhlinen, von, N., Akutsu, M., Ravenhill, B.J., Foeglein, A., Bloor, S., Rutherford, T.J., Freund, S.M.V., Komander, D., and Randow, F. (2012)
    LC3C, Bound Selectively by a Noncanonical LIR Motif in NDP52, Is Required for Antibacterial Autophagy
    Molecular Cell 48: 329–342.
  • Bloor, S., Maelfait, J., Krumbach, R., Beyaert, R. and Randow, F. (2010)
    Endoplasmic reticulum chaperone gp96 is essential for infection with vesicular stomatitis virus.
    Proc Natl Acad Sci U S A 107: 6970-6975.
  • Thurston, T.L., Ryzhakov, G., Bloor, S., von Muhlinen, N. and Randow, F. (2009)
    The TBK1 adaptor and autophagy receptor NDP52 restricts the proliferation of ubiquitin-coated bacteria.
    Nat Immunol 10: 1215-1221.

Group Members

  • Keith Boyle
  • Jack Byrne
  • Ana Crespillo
  • Adely de la Pena Correa
  • Ceara Harper
  • Alexander Heatley
  • Paul Lehner
  • Thomas Mund
  • Prathyush Pothukuchi
  • Chi Jie Matthew Yip

Primary Sidebar

Research Leaders

  • A to G
    • Matteo Allegretti
    • Radu Aricescu
    • David Barford
    • Buzz Baum
    • Anne Bertolotti
    • Tanmay Bharat
    • Mariann Bienz
    • Simon Bullock
    • Albert Cardona
    • Andrew Carter
    • Jason Chin
    • Gerry Crossan
    • Emmanuel Derivery
    • Michel Goedert
    • Julian Gough
    • Ingo Greger
  • H to M
    • Michael Hastings
    • Ramanujan Hegde
    • Richard Henderson
    • Philipp Holliger
    • Leo James
    • Gregory Jefferis
    • Patrycja Kozik
    • Madeline Lancaster
    • Jan Löwe
    • Kate McDole
    • Andrew McKenzie
    • Harvey McMahon
    • Liz Miller
    • Sean Munro
    • Garib Murshudov
  • N to S
    • Kelly Nguyen
    • John O’Neill
    • Lori Passmore
    • Lalita Ramakrishnan
    • Venki Ramakrishnan
    • Felix Randow
    • Jing Ren
    • Christopher Russo
    • Benjamin Ryskeldi-Falcon
    • Julian Sale
    • William Schafer
    • Sjors Scheres
    • Marta Shahbazi
    • John Sutherland
  • T to Z
    • Chris Tate
    • Marco Tripodi
    • Ana Tufegdžić Vidaković
    • Roger Williams
    • Joseph Yeeles
    • Suyang Zhang
    • Marta Zlatic
  • Emeritus
    • Brad Amos
    • Tony Crowther
    • Phil Evans
    • Alan Fersht
    • Michael Gait
    • Rob Kay
    • John Kendrick-Jones
    • John Kilmartin
    • Peter Lawrence
    • Andrew Leslie
    • David Neuhaus
    • Hugh Pelham
    • Daniela Rhodes
    • Murray Stewart
    • Andrew Travers
    • Nigel Unwin
    • Greg Winter
  • LMB Fellows
  • Molecular Immunity Unit

Search

  • Privacy & Cookies
  • Contact Directory
  • Freedom of Information
  • Site Map
Find Us
©2023 MRC Laboratory of Molecular Biology,
Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK. 01223 267000

The MRC is part of UK Research and Innovation

Contact Us